Cytochrome b2 from Bakers' Yeast (L-Lactate Dehydrogenase). A Double-Headed Enzyme
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چکیده
منابع مشابه
Formaldehyde dehydrogenase from bakers' yeast.
In the course of studies of formaldehyde metabolism in yeast (I), an enzyme was found that catalyzed the oxidation of formaldehyde in the presence of glutathione and diphosphopyridine nucleotide. A similar enzyme was discovered independently by Strittmatter and Ball in liver (2). This paper describes the partial purification and some of the properties of yeast formaldehyde dehydrogenase. Some e...
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Cytochrome aa3 was purified 35to 40-fold from submitochondrial particles of commercial bakers’ yeast. The purification procedure involved solubilization of the enzyme with cholate, fractionation with ammonium sulfate, and chromatography on DEAE-cellulose in the presence of Triton x-100. The purified, active enzyme contained approximately 10 nmoles of heme a per mg of protein and was free of oth...
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An apoenzyme, free of flavin mononucleotide, can be prepared by several different methods from cytochrome b,, L( +)-lactate-cytochrome c oxidoreductase (EC 1.1.2.3) of bakers’ yeast. This material shows a complex circular dichroism (CD) spectrum which is strongly dependent on the oxidation state of the heme group. The numerous CD extrema in the region of absorption by the heme group result from...
متن کاملHomology between bakers' yeast cytochrome b2 and liver microsomal cytochrome b5.
The amino-acid sequence of the hemebinding region of bakers' yeast cytochrome b(2) [L-(+)-lactate dehydrogenase, EC 1.1.2.3] has been determined. It shows a strong similarity with the sequence of microsomal cytochrome b(5), and appears to be compatible with the same kind of peptide-chain folding, in agreement with data obtained previously by various physiochemical methods. The comparison shows ...
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ژورنال
عنوان ژورنال: European Journal of Biochemistry
سال: 1974
ISSN: 0014-2956,1432-1033
DOI: 10.1111/j.1432-1033.1974.tb03271.x